We do this when we are trying to collect large numbers of events on samples and are limited by the instrument software as to how many events per file we can have. Biologically, they are not replicates, so it would be begging for a research misconduct inquiry to report an "average" and "standard deviation" on these five. then you might as well concatenate, because the five wells don't control for variation outside of anything other than the instrument collection. reporting distinct values.įirst and foremost, are they really independent wells? e.g., if you take a single sample, process it, stain it, and then only at the end divide the cells into five wells. Several things should be thought about before considering concatenating wells vs. I apologize for giving this question short shrift - it's a very important question and very useful basis for discussion.
This is a question that comes up fairly frequently in our own facility, and serves as a useful springboard for discussions on what constitutes replicates and independent measurements. Over the past few days, I've had some email exchanges with a few people about this, and realized that I should have put much more thought into my original response. Next message: Cryopreservation of live/dead stained samples.Previous message: to concatenate samples for analysis in flowjo.Revisited: to concatenate samples for analysis in flowjo Mario Roederer roederer at nih.gov Revisited: to concatenate samples for analysis in flowjo
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